Quantification of rotenone in seeds of different species of yam bean (Pachyrhizus sp.) by a SPE HPLC-UV method

► We develop a 3 steps method for the accurate quantification of rotenone in yam bean. ► It allows measuring rotenone in seeds from 0.07% up to 1.25% (w/w). ► Accuracy profiles and total error was used as criteria to validate the method. ► It was applied to 17 different accessions of Pachyrhizus erosus and P. ahipa. ► These quantifications lead to results ranging from 1.13 to 2.76 mg/g dry material.