Column-chromatographic extraction and separation of polyphenols, caffeine and theanine from green tea

► High-efficient method for extraction of multiple bioactive substances from green tea. ► Sequential selective resin adsorptions to separate bioactive compounds. ► More than 95% extraction efficiency with 4-fold of solvent. ► High concentration and low impurities of extraction solution. ► Minimum solvent used, minimum solvent concentration and minimum equipment costs.