Monitoring of organophosphorus pesticides in vegetables using monoclonal antibody-based direct competitive ELISA followed by HPLC-MS/MS

► A direct competitive ELISA for organophosphorus pesticides was developed. ► HPLC-MS/MS multi-analytes analysis for organophosphorus pesticides was developed. ► ELISA and HPLC-MS/MS are combined for monitoring of organophosphorus pesticides. ► The methods are rapid, high-throughput and with high-efficiency and low cost.