کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10541816 | 963435 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic response of LAP-activated RAW 264.7 macrophages to the anti-inflammatory property of fungal ergosterol
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Ergosterol is the principal fungal sterol in edible mushrooms and yeast lees in wine. In this study, the immunoregulatory action of ergosterol was evaluated by a proteomic approach using two-dimensional gel electrophoresis to identify proteins with modified expression in LPS-challenged RAW 264.7 macrophages treated with ergosterol. By comparing the proteome signatures, alterations of multiple proteins were observed, including NF-κB cascade-related proteins, Rho family GTPase protein, and cytoskeleton-related proteins. The down-regulation of NF-κB cascade-associated proteins coincided with the inhibition of TNF-α production and COX-2 expression in the ergosterol-treated RAW 264.7 cells. Western blotting analysis indicated that ergosterol inhibits LPS-induced inflammation in RAW 264.7 macrophages by suppressing the NF-κB signalling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 126, Issue 1, 1 May 2011, Pages 207-212
Journal: Food Chemistry - Volume 126, Issue 1, 1 May 2011, Pages 207-212
نویسندگان
Chia-Feng Kuo, Chih-Hsuan Hsieh, Wun-Yuan Lin,