Response surface modelling of the production of structured lipids from soybean oil using Rhizomucor miehei lipase

► Incorporation of PUFAs into soybean oil optimised by Response Surface Methodology. ► The employed method is advantageous because it is solvent-free. ► The molar ratio fatty acids:soybean oil had the greatest influence on incorporation. ► Soybean oil containing EPA and DHA was produced and shows nutritional advantages.