Effects of sampling and extraction on deoxynivalenol quantification

► Among the different steps of mycotoxins analysis the critical one is grain sampling. ► Variance could be linked to DON concentration by the following equation: V = aCb. ► Grinding generated a homogeneous flour sample. ► A longer extraction time with a magnetic agitator improve the extraction rate without increasing the variability. ► Grinding process has an effect on extraction rate.