Extraction, purification and properties of trypsin inhibitor from Thai mung bean (Vigna radiata (L.) R. Wilczek)

► Mung bean seeds grown in Thailand contained high trypsin inhibitory activity. ► A 14 kDa trypsin inhibitor from mung bean seeds was purified and characterized. ► The inhibitor was heat stable and high activity was retained over a wide pH range. ► It was also stable at high salt concentration up to 3%.