Repeated hydrolysis process is effective for enrichment of omega 3 polyunsaturated fatty acids in salmon oil by Candida rugosa lipase

► Omega 3 PUFA were concentrated to 2.4-fold of the initial level. ► Hydrolysis reaction was scaled up without any decrease in the selectivity. ► Short path distillation contributed in recovery of omega 3 PUFA in residue. ► Repeated hydrolysis after distillation improved omega 3 PUFA level further. ► Non-steroselectivity and acyl chain specificity of Candida rugosa lipase were proved.