Development and validation of an HPLC-FLD method for rapid determination of histamine in skipjack tuna fish (Katsuwonus pelamis)

► This study compared and validated two HPLC methods. ► Recoveries greater than 55% could not be observed by HPLC-UV. ► Derivatization with pre-column o-phthalaldehyde was found efficient. ► Good recoveries were obtained at all spiking levels by HPLC-FLD.