کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10543863 | 963557 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of caffeine, theobromine, theophylline, paraxanthine and nicotine in human milk by liquid chromatography with diode array UV detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A liquid chromatography-diode array UV detection (LC-UVDAD) method for the simultaneous determination of the alkaloids Nicotine, Caffeine, Theobromine, Paraxanthine and Theophylline is described. The chromatographic separation was achieved on a LC-18-DB column using 20:80 methanol:buffer (5 mM sodium octane sulphonate, 10 mM citric acid adjusted to pH 5.8 with triethylamine) as mobile phase. The method has been applied to human milk samples. The overall procedure had % recoveries ranging from 60.2 ± 0.6 (theobromine) to 98.6 ± 0.4 (caffeine). The within-day (n = 5) and between-days (n = 5 over 5 days) coefficients of variation in milk ranged from 3.7% (caffeine) to 4.7% (theobromine) and from 5.1% (caffeine) to 6.7% (theobromine). Estimated LOD and LOQ in milk ranged from 8 (caffeine) to 13 (nicotine) ng/ml and from 24 (caffeine) to 34 (nicotine) ng/ml, respectively. Samples naturally contaminated with the target analytes were found.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 93, Issue 1, November 2005, Pages 177-181
Journal: Food Chemistry - Volume 93, Issue 1, November 2005, Pages 177-181
نویسندگان
Antonella Aresta, Francesco Palmisano, Carlo G. Zambonin,