Supercritical carbon dioxide extraction of 2-acetyl-1-pyrroline from Pandanus amaryllifolius Roxb

A comparative evaluation of the extraction of 2-acetyl pyrroline (2-AP) from Pandanus amaryllifolius Roxb. using either solvent extraction (3:1 chloroform:methanol), Likens-Nickerson apparatus or supercritical fluid extraction (SFE) with carbon dioxide extraction was carried out. SFE at 450 bar pressure for 3 h at 60 °C, at a constant flow rate of 0.1 l min−1 of CO2, could extract 2-AP from P. amaryllifolius Roxb. in yields greater than those obtained by solvent extraction or Likens-Nickerson extraction. This extract could find novel applications in food flavouring.