کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10544610 | 963598 | 2005 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a reverse-phase high-performance liquid chromatography method for analyzing trans-resveratrol in peanut kernels
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A reverse-phase high-performance liquid chromatography (HPLC) method for identification and quantification of trans-resveratrol from peanut extracts, using phenolphthalein as internal standard, was developed. The HPLC system consisted of a C18 column (250 l Ã 4.6 i.d. mm, 5 μm particle size), with PDA detection at 307 nm and mobile phase consisting of 0.1% acetic acid in water and 100% acetonitrile. Gradient elution increased acetonitrile linearly from 5% to 41.8% over 23 min (GS = 1.6) followed by an increase of acetonitrile to 77% over 5 min (GS = 7.04), returning to initial conditions over 1 min and held for an additional 5 min with a flow rate of 1.5 ml/min. A lower column temperature resulted in higher peak heights and better baseline separation, therefore 25 °C was selected over 40 or 60 °C to simplify the method. Accuracy, precision, linearity, limit of detection and limit of quantitation were consistent or better than reported previously in the literature for related studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 89, Issue 4, March 2005, Pages 623-638
Journal: Food Chemistry - Volume 89, Issue 4, March 2005, Pages 623-638
نویسندگان
J.L. Rudolf, A.V.A. Resurreccion, F.K. Saalia, R.D. Phillips,