کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550047 | 967012 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HPLC assay for bupropion and its major metabolites in human plasma
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Bupropion is used clinically as an antidepressant and in smoking cessation. As it is metabolised to hydroxybupropion specifically by CYP2B6, bupropion has also been used as a probe to assess CYP2B6 activity. A specific and reproducible HPLC assay has been developed to simultaneously quantify bupropion and its major metabolites hydroxybupropion, threohydrobupropion and erythrohydrobupropion in human plasma. The analysis was performed on an Aqua C18 HPLC column, with a mobile phase consisting of 45:55 of methanol:0.05Â M phosphate buffer (pH 5.5) and simultaneous UV detection at 214Â nm (bupropion metabolites) and 254Â nm (bupropion, internal standard timolol maleate). The assay showed a linear response for bupropion (2.5-250Â ng/mL), threohydrobupropion (5-250Â ng/mL), erythrohydrobupropion (10-250Â ng/mL) and hydroxybupropion (10-1000Â ng/mL). Extraction recovery was reproducible and greater than 55% for each analyte. The inter- and intra-day assay variability (measured as percent coefficient of variation; %CV) was less than 15% for all analytes. Limit of quantification was 2.5Â ng/mL for bupropion, 5Â ng/mL for threohydrobupropion and 10Â ng/mL for hydroxybupropion and erythrohydrobupropion. This assay is more sensitive than currently published methods using HPLC with UV detection for the simultaneous quantitation of bupropion and metabolites and can be used for assessing CYP2B6 activity in vivo following a single dose of bupropion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 823, Issue 2, 5 September 2005, Pages 115-121
Journal: Journal of Chromatography B - Volume 823, Issue 2, 5 September 2005, Pages 115-121
نویسندگان
Katarzyna K. Loboz, Annette S. Gross, John Ray, Andrew J. McLachlan,