کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10550059 | 967012 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of d- and l-enantiomers of methionine and [2H3]methionine in plasma by gas chromatography-mass spectrometry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of d- and l-enantiomers of methionine and [2H3]methionine in plasma by gas chromatography-mass spectrometry Determination of d- and l-enantiomers of methionine and [2H3]methionine in plasma by gas chromatography-mass spectrometry](/preview/png/10550059.png)
چکیده انگلیسی
A method for the stereoselective determination of d- and l-enantiomers of both methionine and [2H3]methionine in rat plasma was developed using gas chromatography-mass spectrometry with selected-ion monitoring (GC-MS-SIM). dl-[2H7]Methionine was used as analytical internal standard to account for losses associated with the extraction, derivatization and chromatography. The amino acids were purified by cation-exchange chromatography using BondElut SCX cartridge and derivatized with HCl in methanol to form methyl ester followed by subsequent N-acylation with optically active (+)-α-methoxy-α-trifluoromethylphenylacetyl chloride to form diastereomeric amide. Quantification was performed by SIM of the molecular-related ions of the diastereomers on the chemical ionization mode. Endogenous l-methionine concentrations in 50 μl of rat plasma were measured with relative intra- and inter-day precision of 4.0 and 6.3%, respectively. The intra- and inter-day reproducibility in the amounts of d- and l-[2H3]methionine determined were in good agreement with actual amount added.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 823, Issue 2, 5 September 2005, Pages 203-208
Journal: Journal of Chromatography B - Volume 823, Issue 2, 5 September 2005, Pages 203-208
نویسندگان
Hiroshi Hasegawa, Yoshihiko Shinohara, Kenji Akahane, Takao Hashimoto,