Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins

► Phosphoprotein changes in A. thaliana leaves were observed after treatments with elicitors or mimicking biotic stresses. ► Phosphoproteins were enriched by means of metal oxide affinity chromatography and resolved by 2-DE. ► Ninety-seven differential spots, representing 75 unique candidate phosphoproteins, were characterized. ► They included proteins involved in energy/carbon metabolism, stress response, defense, RNA processing and cell.