Proteomics of early and late cold shock stress on thermophilic bacterium, Thermus sp. GH5

► The proteomes of the Thermus GH5 after decreasing in temperature were investigated. ► The proteomes of cells grown in cold condition compared to those grown at 75 °C. ► We identified a set of proteins some of which are involved in metabolic processes. ► This organism could be tolerating the stress conditions by changing its metabolism.