Proteomic analysis of advanced colorectal cancer by laser capture microdissection and two-dimensional difference gel electrophoresis

► LCM and 2D-DIGE were used to analyse the proteome of colorectal cancers (CRC). ► The protein expression profile of the primary CRC was indifferent from that of the matched liver metastasis. ► Seventy one proteins were identified to be altered in the primary CRC compared to matched normal colon mucosa. ► Overexpression of ACY1, HSC70, HnRNP I, HnRNP A3, SET, ANP32A and TUFM in CRC has been verified by immunoassays.