Proteomic analysis of 1α,25-Dihydroxyvitamin D3 action on human colon cancer cells reveals a link to splicing regulation

► The action of vitamin D analogues on colon cancer cells was analyzed. ► Nuclear extracts were analyzed with 2D-DIGE and MALDI-TOF-TOF. ► A significant fraction of the identified proteins were involved in RNA processing. ► Another relevant group of proteins was involved in cell adhesion and morphology. ► Vitamin D analogues may play a role in alternative splicing in colon cancer.