Effects of Clostridium difficile Toxin A on the proteome of colonocytes studied by differential 2D electrophoresis

► Our DIGE analysis revealed 8 novel proteins responsive to Toxin A from Clostridium difficile ► Western blot analyses partially confirmed DIGE data ► A glucosyltransferase deficient Toxin A had no detectable effect in colonic cells ► A proteome map for human colonic cells with 186 identified spots is provided