Identification of phosphorylation sites on the E3 ubiquitin ligase UBR5/EDD

► 18 new EDD Phosphorylation sites have been identified. ► S1018 was identified as being phosphorylated, only its homologue in mouse (S1012) has been reported previously. ► S1227 is the first phosphorylation site reported in the zinc finger domain of EDD. ► This work provides a framework to study how basal phosphorylation regulates EDD function.