Proteome expression changes among virulent and attenuated Neospora caninum isolates

► Proteome of virulent and attenuated Neospora caninum isolates are compared by DIGE. ► Comparison across N. caninum isolates revealed protein spots differentially abundant. ► Proteins implicated in parasite motility, invasion and proliferation were identified. ► Protein expression variations could explain differences in Neospora isolate behavior.