Sperm phosphoproteome profiling by ultra performance liquid chromatography followed by data independent analysis (LC-MSE) reveals altered proteomic signatures in asthenozoospermia

► Sixty-six phosphoproteins differentially expressed in severe asthenozoospermia ► Deregulated phosphoproteins reveal pathways important for sperm motility. ► Deregulated proteins correlate with ultra-structure defects in these spermatozoa. ► Signature molecules could be used for diagnosis of asthenozoospermia and improve treatment efficacy.