Tools for probing and perturbing O-GlcNAc in cells and in vivo

- Study of substoichiometric O-GlcNAc modifications requires enrichment from biological samples.
- Metabolic labeling by O-GlcNAc analogues bearing bioorthogonal chemical handles.
- Chemoenzymatic labeling and chemical probes enable protein ID and site mapping.
- Photochemical cross-linking agent enables identification of O-GlcNAc-modified protein binding partners in cells.
- Mass spectrometry-based quantitative proteomics can be applied to O-GlcNAc.