کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10565238 | 971598 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-throughput screens and selections of enzyme-encoding genes
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>106 gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 9, Issue 2, April 2005, Pages 210-216
Journal: Current Opinion in Chemical Biology - Volume 9, Issue 2, April 2005, Pages 210-216
نویسندگان
Amir Aharoni, Andrew D Griffiths, Dan S Tawfik,