کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10565386 971679 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Photoswitchable fluorescent proteins: ten years of colorful chemistry and exciting applications
ترجمه فارسی عنوان
پروتئین فلورسنت عکسبرداری: ده سال از شیمی رنگارنگ و کاربردهای هیجان انگیز
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی
Reversibly photoswitchable fluorescent proteins (RSFPs) are fluorescent proteins whose fluorescence, upon excitation at a certain wavelength, can be switched on or off by light in a reversible manner. In the last 10 years, many new RSFPs have been developed and novel applications in cell imaging discovered that rely on their photoswitching properties. This review will describe research on the mechanisms of reversible photoswitching and recent applications using RSFPs. While cis-trans isomerization of the chromophore is believed to be the general mechanism for most RSFPs, structural studies reveal diversity in the details of photoswitching mechanisms, including different effects of protonation, chromophore planarity, and pocket flexibility. Applications of RSFPs include new types of live-cell superresolution imaging, tracking of protein movements and interactions, information storage, and optical control of protein activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 17, Issue 4, August 2013, Pages 682-690
نویسندگان
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