Making connections - strategies for single molecule fluorescence biophysics

- The single-molecule approach yields exciting insights for many biomolecular applications.
- There are significant challenges to achieve main-stream single-molecule measurements.
- New labelling chemistries enable multiple tagged molecules in vitro and in live cells.
- Single-molecule pull-down expands the toolbox complementing co-immunoprecipitation.
- Breaking the single-molecule concentration barrier is within reach.