Profiling and inhibiting reversible palmitoylation

► New methods allow direct enrichment of palmitoylated proteins for LC-MS annotation. ► SILAC proteomics methods enhance the annotation of low abundance palmitoyl proteins. ► Alkynyl fatty acids facilitate pulse-chase labeling for measuring palmitoylation dynamics. ► Thioesterase inhibitors are promising new tools to dissect palmitoylation dynamics.