کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10574 692 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: Morphology, cytotoxicity, and oxidative stress
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: Morphology, cytotoxicity, and oxidative stress
چکیده انگلیسی

Recent studies demonstrated that Co2+ and Cr3+ ions induced cell mortality, TNF-α secretion, and oxidation of proteins in macrophages. However, little is known about the effects of corrosion products on the osteogenic cells, which have a crucial role in controlling bone remodeling. The aim of the present study was to investigate the effect of Co2+ (0–10 ppm) and Cr3+ (0–150 ppm) on human MG-63 osteoblast-like cells in term of cytotoxicity and oxidative stress. Microscopic analysis demonstrated changes in shape, size, and number of cells. Co2+ had a greater effect on these parameters than Cr3+. Cell counting showed a significant decrease in the number of MG-63 osteoblasts in a time- and dose-dependent manner, with Co2+ more toxic than Cr3+. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis also showed a decreased cellular activity in presence of Co2+ and Cr3+ ions. Oxidized and nitrated proteins, two markers of oxidative stress, were detected as single bands and revealed time- and dose-dependent protein modifications. We also studied the expression of three antioxidant enzymes. The expression of heme oxygenase-1 was increased by both ions after 24 h, before decreasing gradually thereafter. Glutathione peroxidase expression was also increased in a concentration- and time-dependent manner by both Co2+ and Cr3+ ions. Co2+ decreased catalase expression while Cr3+ increased it in a dose- and time-dependent manner. In conclusion, this study demonstrated that Cr3+ and Co2+ have a cytotoxic effect on MG-63 osteoblasts and have the potential to modify their redox state.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 27, Issue 18, June 2006, Pages 3351–3360
نویسندگان
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