کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10582509 | 981047 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorescent indicator displacement assay of ligands targeting 10 microRNA precursors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Fluorescent indicator displacement assay of ligands targeting 10 microRNA precursors Fluorescent indicator displacement assay of ligands targeting 10 microRNA precursors](/preview/png/10582509.png)
چکیده انگلیسی
Fluorescent indicator displacement (FID) assay is a rapid and convenient assay for identifying new ligands that bind to the target molecules. In our previous studies, we have shown that a series of 2,7-diaminoalkoxy xanthone and thioxanthone derivatives can be used as fluorescent indicators for detecting the interaction between RNA and a ligand. The xanthone and thioxanthone fluorochromes showed efficient fluorescence quenching upon binding to target RNA. Subsequent displacement of the bound-fluorochrome with a ligand that binds more strongly to the target RNA led to the recovery of the fluorescence by releasing the fluorochrome from RNA. Here we report a pilot screening of a chemical library that contains 9600 structurally diverse compounds for molecules that bind to a specific miRNA precursor using the FID assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 22, 15 November 2013, Pages 7101-7106
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 22, 15 November 2013, Pages 7101-7106
نویسندگان
Asako Murata, Yasue Harada, Takeo Fukuzumi, Kazuhiko Nakatani,