Synthesis and anti-hepatitis B virus activity of C4 amide-substituted isosteviol derivatives

Among several C4 amide-substituted isosteviol derivatives, IN-4 exhibited inhibitory activity against secretion of HBsAg and HBeAg as well as inhibition of HBV DNA replication. Its action mechanism was further elucidated in HBV-transfected Huh7 cells.