Photoligation of self-assembled DNA constructs containing anthracene-functionalized 2′-amino-LNA monomers

Efficient synthesis of a novel anthracene-functionalized 2′-amino-LNA phosphoramidite derivative is described together with its incorporation into oligodeoxynucleotides. Two DNA strands with the novel 2′-N-anthracenylmethyl-2′-amino-LNA monomers can be effectively cross-linked by photoligation at 366 nm in various types of DNA constructs. Successful application of three differently functionalized 2′-amino-LNA monomers in self-assembled higher ordered structures for simultaneous cross-linking and monitoring of assembly formation is furthermore demonstrated.