Synthesis of ethynylbenzene-substituted glycol as a versatile probe for labeling oligonucleotides

Positron emission tomography (PET) is a highly sensitive quantitative imaging technique for studying molecular pathways and interactions in vivo. This imaging technique plays a key role in drug discovery, pharmacokinetics, pharmacodynamics, and assessing in vivo distribution. In this study, we designed an ethynylbenzene-substituted glycol (ME) as a versatile probe for PET labeling of oligonucleotides through a click reaction.