Effect of Chinese medicine alpinetin on the structure of human serum albumin

Alpinetin is the bioactive component of a traditional Chinese medicine. This study is designed to examine the effect of alpinetin on the solution structure of HSA using UV-visible spectroscopic, circular dichroism (CD), Fourier transform infrared (FT-IR), and fluorescence spectroscopic methods at four temperatures under physiological conditions. Attempts were made to investigate the binding mechanism of alpinetin to HSA and the effect of alpinetin on the protein secondary structure. The partial binding parameters of the reaction were calculated through SGI FUEL workstations.