کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10592362 981788 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
TACN-based cationic lipids with amino acid backbone and double tails: Materials for non-viral gene delivery
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
TACN-based cationic lipids with amino acid backbone and double tails: Materials for non-viral gene delivery
چکیده انگلیسی
Cationic lipids have become an efficient type of non-viral vectors for gene delivery. In this Letter, four cationic lipids containing 1,4,7-triazacyclononane (TACN) headgroup, glutamic/aspartic acid backbone and dioleyl tails were designed and synthesized. The TACN headgroup gives these lipids excellent pH buffering capacities, which were higher than branched 25 kDa PEI. Cationic liposomes prepared from these lipids and DOPE showed good DNA affinity, and full DNA condensation was found at N/P ratio of 3 via agarose gel electrophoresis. The lipoplexes were characterized by dynamic light scattering (DLS) assay, which gave proper particle sizes and zeta-potentials for transfection. In vitro gene transfection results in two cell lines reveal that TAN (with aspartic acid and amide bond in the structure) shows the best transfection efficiency, which is close to commercially available transfection agent Lipofectamine 2000.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 24, Issue 7, 1 April 2014, Pages 1771-1775
نویسندگان
, , , , , ,