کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10595719 | 981876 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolic labeling of fucosylated glycoproteins in Bacteroidales species
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Metabolic labeling of fucosylated glycoproteins in Bacteroidales species Metabolic labeling of fucosylated glycoproteins in Bacteroidales species](/preview/png/10595719.png)
چکیده انگلیسی
Members of the Bacteroidales order are among the most abundant gram-negative bacteria of the human colonic microbiota. These species decorate their cell-surface glycoproteins with fucosylated glycans, which are believed to play important roles in host intestinal colonization. Currently, there is no method for the enrichment of these glycoproteins for their identification. Here, we describe a chemical approach directed toward labeling and detecting fucosylated glycoproteins from cultured Bacteroidales species, namely Bacteroides fragilis and Parabacteroides distasonis. We treated these bacteria with an alkyne-bearing fucose analog, which is metabolically integrated into the bacterial surface fucosylated glycoproteins. The alkyne-tagged glycoproteins can then react with azide-bearing biophysical probes via bioorthogonal click chemistry for detection or glycoproteomic analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 21, Issue 17, 1 September 2011, Pages 4989-4992
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 21, Issue 17, 1 September 2011, Pages 4989-4992
نویسندگان
Christen Besanceney-Webler, Hao Jiang, Wei Wang, Anthony D. Baughn, Peng Wu,