Synthesis and incorporation into DNA fragments of the artificial nucleobase, 2-amino-8-oxopurine

The synthesis of 2-amino-9-(2-deoxy-β-d-ribofuranosyl)-7,8-dihydro-8-oxo-purine (dJ) and its incorporation into DNA via a convertible phosphoramidite are reported. The thermal stability of heteroduplexes containing J has been studied by UV thermal-denaturation experiments.