N,N,N-Trimethyl chitosan nanoparticles for controlled intranasal delivery of HBV surface antigen

► Size controllable formulation of N-trimethyl chitosan NPs is reported. ► Ionic gelation method is utilized to produce stable HBsAg loaded NPs. ► HBsAg loading efficiency and capacity is found to be higher than standard. ► The controlled release of HBsAg is noticed for 43 days without any burst release. ► The released HBsAg is highly stable without any polymer destruction.