Lactarius rufus (1→3),(1→6)-β-d-glucans: Structure, antinociceptive and anti-inflammatory effects

► Two β-d-glucans from wild mushroom Lactarius rufus were structurally characterized. ► β-Glucans inhibited significantly inflammatory pain caused by formalin injection. ► β-Glucans branching degree seems to be important for their antinociceptive effect. ► Linear β-glucan inhibitory effect was more marked in response to neurogenic pain. ► FSHW with higher branching degree inhibited more significantly inflammatory pain.