S-protected thiolated chitosan: Synthesis and in vitro characterization

► Synthesis of a novel mucoadhesive thiolated chitosan with protected thiol groups. ► The novel conjugate exhibited promising mucoadhesive features. ► In vitro cytotoxicity of the new conjugate was evaluated and found to be non-toxic. ► Swelling behavior of the polymer decreased with the increase of protection. ► Enhanced cross linking within the novel conjugate resulted in improved stability.