Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides

► This report describes the highly specific capture of glycopeptides employing chitosan-GMA-APB nanospheres. ► These nanospheres have a diameter of 20-100 nm and were synthesized for this work. ► MALDI-TOF-MS combined with use of these nanospheres is a simple and rapid approach for enrichment of glycopeptides. ► The binding affinity of boronic acid and glycopeptides is pH and solvent dependent.