Purification, characterization and anticoagulant activity of the polysaccharides from green tea

► Four major polysaccharide fractions were purified with DEAE sepharose CL-6B. ► The average molecular weight was analyzed by HPGPC. ► Monosaccharide composition of polysaccharide fractions were analyzed HPAEC-PAD. ► TPS-4 showed significant anticoagulant activity in APTT and TT assay.