In vitro evaluation of mucoadhesion and permeation enhancement of polymeric amphiphilic nanoparticles

► OCMCS was investigated for the mucoadhesion and permeation enhancement at neutral pH. ► OCMCS nanoparticles showed high adhesion tendency onto carp intestinal mucosa. ► OCMCS nanoparticles promoted FD4 transport through excised carp intestinal mucosa. ► The improvement of permeability was further verified via Caco-2 cell monolayer. ► Cytotoxicity studies in Caco-2 monolayers verified the safety of the delivery system.