Optimization of ultrasonic-assisted extraction of water-soluble polysaccharides from Boletus edulis mycelia using response surface methodology

► Extraction of water-soluble polysaccharides from Boletus edulis mycelia. ► Ultrasonic and response surface methodology were used. ► Ratio of dried mycelia to water, extraction time and ultrasonic temperature were discussed. ► Model was set up to optimization extraction of water-soluble polysaccharides. ► The best extraction methods were 56 °C, 1:55 of ratio of dried mycelia to water, and 8.4 min.