Effect of anticoagulative sulfated polysaccharide purified from enzyme-assistant extract of a brown seaweed Ecklonia cava on Wistar rats

► We purified a sulfated polysaccharide from brown seaweed Ecklonia cava. ► We investigated anticoagulant activity in vitro and in vivo. ► Anticoagulant activity comparable to that of a commercial fucoidan. ► The sulfated polysaccharide extended the coagulation time in Wistar rats.