Effect of chitosan on membrane permeability and cell morphology of Pseudomonas aeruginosa and Staphyloccocus aureus

► We examined the modes of action of chitosan on P. aeruginosa and S. aureus. ► Chitosan resulted in leakage of cell inclusions (enzymes, DNA, proteins and ions). ► Chitosan increased the membrane permeability by adhering to cell membranes. ► TEM verified that S. aureus is more sensitive than P. aeruginosa to chitosan.