High production of cellulose degrading endo-1,4-β-d-glucanase using bagasse as a substrate from Bacillus subtilis KIBGE HAS

► Bagasse was used as a source for the production of cellulose degrading enzyme. ► Maximum production of endo-1,4-β-d-glucanase was obtained at pH 7.0. ► Maximum endo-1,4-β-d-glucanase production was obtained after 48 h incubation. ► Endo-1,4-β-d-glucanase showed its activity in wide temperature range with optimum of 60 °C. ► Ammonium nitrate was used as a nitrogen source for maximum enzyme production.