کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10603463 | 982479 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activity of chitin deacetylase from Colletotrichum gloeosporioides on chitinous substrates
ترجمه فارسی عنوان
فعالیت کیتین داس تییلاز از کلوتوتوریوم کورئوسپورویید بر روی زیره های کتین
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
Production of chitin deacetylases from the phytopathogenic fungus Colletotrichum gloeosporioides was successfully achieved by submerged fermentation. The highest specific activity of 0.018 U mgâ1 of protein was obtained after 96 h of cultivation at pH 6 and 28 °C. Two bands with molecular weights of 35 kDa and 170 kDa determined with SDS-PAGE displayed deacetylase activities as detected in the zymograms. Reacetylated commercial chitosan (52% acetylation degree) was used as substrate for the extracellular crude extract in order to estimate the kinetic parameters of acetate production as undirected deacetylation measurement. The highest acetate production of 12.8 μmol mLâ1 was obtained using 7.5 mg mLâ1 of substrate. The produced enzyme from C. gloeosporioides achieved up to 25% deacetylation of a chitin substrate (hydrolyzed biological chitin) having 80% degree of acetylation, MW of 102 Ã 103 g molâ1 and a crystallinity index of ca. 60%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 96, Issue 1, 1 July 2013, Pages 227-232
Journal: Carbohydrate Polymers - Volume 96, Issue 1, 1 July 2013, Pages 227-232
نویسندگان
Neith Pacheco, Stéphane Trombotto, Laurent David, Keiko Shirai,