کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10603490 | 982479 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Studies on production of fructo-oligosaccharides (FOS) by gamma radiation processing of microbial levan
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Microbial levan, a natural polymer of fructose, was produced and purified by alcohol precipitation from culture supernatants of Bacillus megaterium type 1 grown in an optimized liquid sucrose medium. GPC analysis showed that the yield of the major fraction of levan having molecular weight ~5000Â D increased with increase in sucrose concentration in the broth. Levan subjected to 60Co-gamma radiation as well as acid hydrolysis was investigated by rheometry, UV-visible spectrophotometry and gel permeation chromatography (GPC) techniques. Unlike most of the polysaccharides, levan powder exhibited good radiation degradation stability up to 150Â kGy. Gamma irradiation of 10% levan aqueous solution at 250Â kGy yielded 63.0% fructo-oligosaccharide (FOS) with an average molecular weight of 1250Â D. Acid hydrolysis of levan using 0.5Â N HCl for 60Â min treatment time gave rise to the desired FOS with lower yield (23.1%) as compared to that obtained in gamma radiolysis process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 96, Issue 1, 1 July 2013, Pages 365-370
Journal: Carbohydrate Polymers - Volume 96, Issue 1, 1 July 2013, Pages 365-370
نویسندگان
N. Jalan, Lalit Varshney, Nilanjal Misra, Jhimli Paul, D. Mitra, D.D. Rairakhwada, Z. Bhathena, Virendra Kumar,