pH effects on the hyaluronan hydrolysis catalysed by hyaluronidase in the presence of proteins. Part III. The electrostatic non-specific hyaluronan-hyaluronidase complex

- Electrostatic complexes are formed between hyaluronan and hyaluronidase at pH 3-10.
- At 6 °C, sedimentable slightly charged and soluble highly charged complexes exist.
- One HAase molecule is associated with 23-71 HA carboxyl groups, depending on pH.
- The pI value for hyaluronidase has been estimated to 5.3.
- Positive patches on the HAase molecule allow the complex formation at pH 7.