Self-assembled nanoparticles of methotrexate conjugated O-carboxymethyl chitosan: Preparation, characterization and drug release behavior in vitro

- Methotrexate was conjugated to O-carboxymethyl chitosan by amide linkage.
- OCM-chitosan-MTX conjugate could form nanoparticles by self-assembly.
- MTX exhibited in vitro sustained release from OCM-chitosan-MTX nanoparticles.